Eurizon.eu

Organization: Uppsala University

Location: Uppsala, Sweden

Field: Clinical Pharmacology

Requirements:

The applicant should have an academic degree e.g. MSc Pharm, or MSc molecular Biology, or similar. Experience from cell cultivation and working under aseptic condition are considered an additional qualification. Previous experience in experimental research is a merit. Skills in oral and written english is necessary.
The application should include a curriculum vitae, copies of exams, degrees and grades, a copy of your Master thesis (or a draft thereof), references, a short description of the applicant and his/her experience. Letter(s) of recommendation may be included

Abstract:

To investigate the activity of different anticancer drugs

Description:

To investigate the activity of different anticancer drugs on
1. tumor cells cultivated in spheroids, i.e. small micro-tumors (0.1-1 mm) with a hypoxic core
2. tumor cells cultivated under hypoxic conditions in comparison to normoxic conditions, with special attention to drugs with potentially increased hypoxic activity and their mechanism.
 
Work plan: To generate hypoxic growth environment to the cells the complete experiments, from addition of drug until end of incubation, will be performed in a hypoxic chamber. The cell’s sensitivity towards a panel of standard chemotherapeutic agents will be analyzed with the fluorometric microculture cytotoxicity assay (FMCA), which measures cell viability on the basis of fluorescein diacetate hydrolysis in cells with intact plasma membranes. The mechanistic investigation will be performed using gene expression analysis and specific kits for molecular events. Isolated mRNA from hypoxic vs normoxic cancer cells, with or without drug exposure, will be analyzed with Affymetrix technique using the Uppsala Array Platform. Cancer cells in spheroids will be cultivated using commercially available tools, e.g. microtiter plates from Scivax.  
 
The application should include a curriculum vitae, copies of exams, degrees and grades, a copy of your Master thesis (or a draft thereof), references, a short description of the applicant and his/her experience. Letter(s) of recommendation may be included.
For more information, please contact Rolf Larsson, phone +4618-6115098, e-mail: rolf.larsson@medsci.uu.se  or Anna Foyer, personal coordinator, phone +4618-611 43 92, anna.foyer@medsci.uu.se. Union representatives are Anders Grundström, Saco, +46 (0)18-471 5380, Carin Söderhäll, TCO/ST, +46 (0)18-471 1996 and Stefan Djurström Seko, +46 (0)18-471 3315.
 

Deadline: 08-02-2011

Contacts:

Link: http://www.personalavd.uu.se/ledigaplatser/193PhD.html

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